Core Services

Contact

MitoCare Center for Mitochondrial Image Research & Diagnostics

1020 Locust Street
Jefferson Alumni Hall, Suite 527
Philadelphia, PA 19107

MitocareCenter@jefferson.edu

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Are you interested in accessing our shared facilities to study mitochondria?

View our shared resources or reach out directly to our leadership to discuss your projects.

Mitochondrial Bioenergetics

Seahorse XFe24

The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples. With Agilent Seahorse XFe24 Analyzers you can measure the OCR and ECAR of live cells in a 24-well plate format without the need of cellular trypsinization. The sensitity of Seahorse is unparalleled and has been widely embraced by the bioenergetics community.

Contact: Erin.Seifert@jefferson.edu

Confocal/Multiphoton Microscopy

Zeiss LSM780 NLO

Explore fundamental mechanisms in functional tissue by the deep penetration achieved by multiphoton microscopy. With low absorption and next to no scattering loss, you can measure fluorescence at depths of 1 mm with subcellular resolution. Plus, the pulsed ultrafast infrared laser is able to excite dyes only at the focus point, preserving the rest of the specimen. The LSM780 is an optically perfect system from 350 to 1100 nm and will take your biomedical research to the next step.

Contact: David.Weaver@jefferson.edu

Transmission Electron Microscopy

FEI Tecnai 12

Using electron optics in lieu of light, allows low nanometer to subnanometer resolution insights to the ultrastructure of fixed specimen derived from tissue, cells or subcellular preparations that have been processed for enhanced electron scattering by the structures of interest and for resisting high vacuum. 2D insights are delivered by a Thermo/FEI Tecnai 12 digital transmission electron microscope (TEM), featuring 120 kV maximum electron beam acceleration voltage and a bottom-mounted AMT BioSprint 12 megapixel High Definition CCD camera. We offer TEM imaging, sample processing (e.g. positive staining, resin-embedding of cells/tissue, negative staining of vesicles or purified proteins) and sectioning services as well as consultations in ultrastructure-directed experimental design and analysis.

Contact: Gyorgy.Csordas@jefferson.edu

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