Metabolomic
Contact
Name:
Zachary T. Schug, PhD
Position:
Associate Professor, Department of Pharmacology, Physiology & Cancer Biology
Organization:
Sidney Kimmel Medical College
Room 804 BLSB
Email:
Zachary.Schug@jefferson.edu
Contact Number(s):
Contact
Name:
Dzmitry Mukha, PhD
Mission
Our facility aims to support scientific innovation through robust and reliable metabolomics.
Expertise & Focus
Our team has decades of experience in LC-MS/MS based metabolomics applied to human health and disease, with specific expertise in cancer metabolism.
Services
- Polar Metabolites: 319 analytes / 475 transitions in every sample run, including ATP/ADP, NADH/NAD+, NADPH/NADP+, acetyl-CoA, SAM/SAH, G1P, G6P, acyl-carnitines, and TMAO
- Fatty Acids: over 70 fatty acids
- Absolute Quantitation: >60 metabolites
- Custom: drugs, inhibitors, and test compounds
Equipment
SCIEX QTRAP® 5500 mass spectrometer and Shimadzu LC-20 Prominence HPLC
Retsch CryoMill: Tissue processing at liquid nitrogen temperatures
Sample Types
- Tissues, organs, and tumors (any species)
- 2D/3D cultured cells (organoids, spheroids)
- Bacteria (stool/chyme)
- Biological fluids (blood, serum, plasma, CSF, urine)
Deliverables
Users will receive a Raw Data and a detailed Summary Report including metabolite names, transitions, retention times, peak areas, peak heights, ratios, and other relevant information.
User guides are also available for further data interpretation and understanding.
Support
- Pre-project consultation
- Experimental design & grant support
- Statistical analyses & visualizations
- Co-authorship / Collaboration opportunities