Metabolomics

The Thomas Jefferson University Metabolomics Service Center aims to support scientific innovation through robust and reliable metabolomics.

Our team has decades of experience in LC-MS/MS based metabolomics applied to human health and disease, with specific expertise in cancer metabolism.

The Thomas Jefferson University Metabolomics Service Center provides targeted LC–MS/MS analysis of polar mammalian metabolites and free fatty acids. Our polar metabolite panel spans amino acids, glycolytic and TCA cycle intermediates, redox cofactors (including NADH and NADPH), nucleosides and nucleotide phosphates, coenzyme A species and their derivatives (e.g., acetyl‑CoA, lactoyl‑CoA), short‑chain fatty acids, neuromediators, and additional physiologically relevant metabolites, as well as common food additives.

We support analysis of cultured cells, media, body fluids, and tissues. Depending on sample type and expected concentrations, each sample can be profiled for more than 300 metabolites in a single run. Absolute quantification is available for a defined subset of analytes.

• Polar Metabolites: >300 analytes / >475 transitions in every sample run, including ATP/ADP, NADH/NAD+, NADPH/NADP+, acetyl-CoA/CoA, SAM/SAH, G1P, G6P, acyl-carnitines, and TMAO.
• Fatty Acids: over 70 fatty acids.
• Absolute Quantitation: >60 metabolites.
• Custom analysis: drugs, inhibitors, and test compounds.

• Tissues, organs, and tumors (any species)
• 2D/3D cultured cells (organoids, spheroids)
• Bacteria (stool/chyme)
• Biological fluids (blood, serum, plasma, CSF, urine)

Users will receive a Raw Data and a detailed Summary Report including metabolite names, transitions, retention times, peak areas, peak heights, ratios, and other relevant information.

User guides are also available for further data interpretation and understanding.