The Jefferson cryo-Electron Microscopy Core (JEMiCo) provides access to a state-of-the-art Glacios 200 kV transmission cryo-Electron Microscope equipped with Falcon 4 direct electron detector. The Glacios uses the same Autoloader for automated and contamination-free loading of cassettes commonly found on the Titan Krios 300 kV. Samples screened on a Glacios can be rapidly transferred to a Krios for ultimate high-resolution data acquisition. Besides, configured with the new Falcon 4, the Glacios is also a powerful microscope for rapid high-resolution Single-Particle Analysis (SPA) data acquisition, capable of achieving resolution beyond 3.5 Å resolution.
Integrated Structural Biology
Contact
Name:
Dmitry Temiakov, PhD,
Contact
Name:
Erik Debler, PhD
Contact
Name:
Ashok Nayak, PhD
Contact
Name:
Hideharu Hashimoto, PhD
Position:
X-Ray Crystallography Facility Manager
Contact Number(s):
Mission, Goals & Capabilities
This facility supports SKCCC research programs with cutting-edge technologies and services in structural biology, including cryo-electron microscopy, x-ray crystallography, characterization of biological macromolecules, and analysis of their interactions.
Major Equipment
Cryo-Electron Microscopy
Reserve equipment through iLab
- ThermoScientific 200kV Glacios transmission electron microscope equipped with a Falcon 4 direct electron detector
- Vitrobot 5 for cryo-EM sample preparation
- PELCO easiGlow™ Glow Discharge system
- Grid clipping and loading stations
- Users training in vitrification of biological specimens
- User training and support in screening cryo-grids and cryo-EM data collection
- Rapid collection of movies in electron-event representation (EER) format (>150 movies/hour)
- Assisted vitrification and grid clipping of biological specimens for inexperienced users
- Assisted data collection and analysis for inexperienced users
X-ray Crystallography
Reserve equipment through iLab
- Rigaku MicroMax-007HF microfocus rotating anode x-ray generator dual equipped for data collection from macromolecular crystals and Small Angle X-Ray Scattering (SAXS). The left port of this instrument is equipped with high focusing mirrors and an ultrafast PILATUS 200K Hybrid Pixel Array Detector and a X-stream 2000 cryogenic system, while the right port hosts a BioSAXS-2000 2D Kratky SAXS system
- Formulatrix NT8 robot crystallization robot for high-throughput crystallization screening
- Microscope equipped with digital camera for crystal viewing and image capture
Molecular Characterization
Reserve equipment through iLab
- Biacore 3000 Surface plasmon resonance
- Low volume Nano-Isothermal Titration Calorimeter (Nano-ITC)
- Beckman Coulter XL-1 Analytical Ultracentrifuge (AUC)
- Jasco J-810 circular dichroism spectropolarimeter (CD)
Services
User training and support in instrumentation, sample preparation, data collection, determining three-dimensional atomic structures, macromolecular structure-function studies, and biophysical characterization.