Cryo-Electron Microscopy
Contact
Name:
Dmitry Temiakov, PhD
Mission, Goals, Capabilities
The Jefferson cryo-Electron Microscopy Core (JEMiCo) provides access to a state-of-the-art Glacios 200 kV transmission cryo-Electron Microscope equipped with Falcon 4 direct electron detector. The Glacios uses the same Autoloader for automated and contamination-free loading of cassettes commonly found on the Titan Krios 300 kV. Samples screened on a Glacios can be rapidly transferred to a Krios for ultimate high-resolution data acquisition. Besides, configured with the new Falcon 4, the Glacios is also a powerful microscope for rapid high-resolution Single-Particle Analysis (SPA) data acquisition, capable of achieving resolution beyond 3.5 Å resolution.
Major Equipment
Reserve equipment through EquipMe
- Thermo Scientific™ Glacios™ Cryo-Transmission Electron Microscope (Cryo-TEM) equipped with Falcon 4 Direct Electron Detector
- GPU-workstation for on-the-fly analysis of micrographs
- Proxy-PC connected to a 2.2 petabytes research storage area for data back-up
- Thermo Scientific Vitrobot Mark IV System
- PELCO easiGlow™ Glow Discharge system
- Grid clipping and loading stations
- Several liquid nitrogen dewars and cryogenic tools
Services
- Users training in vitrification of biological specimens
- User training and support in screening cryo-grids and cryo-EM data collection
- Rapid collection of movies in electron-event representation (EER) format (>150 movies/hour)
- Assisted vitrification and grid clipping of biological specimens for inexperienced users
- Assisted data collection and analysis for inexperienced users