Cryo-Electron Microscopy

Mission, Goals, Capabilities

The Jefferson cryo-Electron Microscopy Core (JEMiCo) provides access to a state-of-the-art Glacios 200 kV transmission cryo-Electron Microscope equipped with Falcon 4 direct electron detector. The Glacios uses the same Autoloader for automated and contamination-free loading of cassettes commonly found on the Titan Krios 300 kV. Samples screened on a Glacios can be rapidly transferred to a Krios for ultimate high-resolution data acquisition. Besides, configured with the new Falcon 4, the Glacios is also a powerful microscope for rapid high-resolution Single-Particle Analysis (SPA) data acquisition, capable of achieving resolution beyond 3.5 Å resolution.

Major Equipment

Reserve equipment through EquipMe

  • Thermo Scientific™ Glacios™ Cryo-Transmission Electron Microscope (Cryo-TEM) equipped with Falcon 4 Direct Electron Detector
  • GPU-workstation for on-the-fly analysis of micrographs
  • Proxy-PC connected to a 2.2 petabytes research storage area for data back-up
  • Thermo Scientific Vitrobot Mark IV System
  • PELCO easiGlow™ Glow Discharge system
  • Grid clipping and loading stations
  • Several liquid nitrogen dewars and cryogenic tools


  • Users training in vitrification of biological specimens
  • User training and support in screening cryo-grids and cryo-EM data collection
  • Rapid collection of movies in electron-event representation (EER) format (>150 movies/hour)
  • Assisted vitrification and grid clipping of biological specimens for inexperienced users
  • Assisted data collection and analysis for inexperienced users