HPLC/Biochemistry Core
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Mission, Goals, Capabilities
The mission of the Jefferson HPLC Core facility is to provide high-sensitivity and fast throughput analytic services for the quantification (pM sensitivity) of aminergic neurotransmitters and their metabolites from tissue samples. We are also capable of detecting and measuring (at pM concentrations ATP, ADP and AMP from tissue.
We hope to add additional capabilities to measure other amino acid transmitters in the future. We can also develop any other HPLC assay per an investigators requirements.
The facility will accept samples from all Jefferson University Investigators as well as from outside institutions. Our goal is to have a turnaround of no more than 2 weeks from the time of sample submission.
Major Equipment
2: ESA HPLC with autosamplers and UltiMate 3000 Electrochemical Detectors. Each HPLC runs on Chromeleon 7.2 Chromatography Data System Software.
Depending on need, we have the capability of adding UV detectors.
Services Provided
2: ESA HPLC with autosamplers and UltiMate 3000 Electrochemical Detectors. Each HPLC runs on Chromeleon 7.2 Chromatography Data System Software.
Depending on need, we have the capability of adding UV detectors.
- Detection of Dopamine (DA) and its metabolites Homovanillic acid (HVA) and 3,4-Dihydroxyphenylacetic acid (DOPAC), as well as its precursor, L-DOPA.
- Detection of Norepinephrine (NE).
- Detection of Epinephrine.
- Detection of serotonin (5-HT) and its metabolite 5-hydroxyindolacetic acid (5-HIAA).
- Detection of ATP, ADP and AMP from tissue samples.