Flow Cytometry

Mission, Goals, Capabilities

The Kimmel Cancer Center Flow Cytometry facility is the only shared facility of its kind on the TJU campus to provide state-of-the-art fluorescence activated cell sorting and analysis capabilities. In addition to the Kimmel Cancer Center (KCC) members, many investigators in several Departments through the campus utilize the facility on regular basis. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics.  The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples.

The services provided by the facility are tailored to suit experimental designs in a variety of fields. We provide consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting KCC and TJU campus-wide investigators performing various research projects directly related to the understanding of the biology and treatment of cancer. The facility supports projects from investigators in Biochemistry,  Cancer Biology,  Dermatology, Immunology & Microbiology, Medicine, Neurology, Pathology, Pharmacology, and Surgery, and many other scientific fields.

Major Equipment

  • BD Aria SORP high speed cell sorter with bio-safety hood (two lasers, 8 colors)
  • Moflo legacy high speed cell sorter (four lasers, 9 colors)
  • BD LSR II multicolor analyzer (four laser, 13 colors)
  • BD Calibur multicolor analyzer (two lasers, 4 color)
  • One PC workstation with FlowJo v10
  • One Mac workstation with FlowJo v8

Services

  • Multi-color flow panel analysis
  • Multi-color cell sorting (including sort into 15ml, 5ml, 1.5ml tube, 6 well, 12 well, 24 well, 48 well and 96 well plate, onto slide)
  • Consulting for experiment design, analyzing data
  • Training for new users
  • Teaching lectures for Jefferson Graduate school and Bioscience Technology program