Flow Cytometry & Human Immune Monitoring
Name:
Larry Harshyne, PhD
Name:
Luis Sigal, DVM, PhD
Name:
Brian Montoya, PhD
Position:
Facility Manager, Flow Cytometry
Email:
Brian.Montoya@jefferson.edu
Name:
Brenda Vidals
Position:
Lab Coordinator, Flow Cytometry
Email:
Brenda.Vidals@jefferson.edu
Name:
Shannon Lynch
Position:
Research Assistant, Human Immune Monitoring
Email:
Shannon.Lynch@jefferson.edu
Contact
Name:
Flow Cytometry
233 S. 10th Street
BLSB 606
Philadelphia, PA 19107
Contact Number(s):
Name:
Human Immune Monitoring
233 S. 10th Street
BLSB 822
Philadelphia, PA 19107
Contact Number(s):
Mission, Goals, Capabilities
The Sidney Kimmel Comprehensive Cancer Center Flow Cytometry and Human Immune Monitoring facility provides state of the ary fluorescence activated cell sorting and analysis capabilities, extracellular vesicle enrichment and analysis workflow and full service immune profiling for clinical and translational scientist. The most commonly used flow cytometry applications include cell surface staining, blood immune cell phenotyping, intracellular cytokine detection, phosphorylation and intracellular signaling pathway activation, transfection efficiency, apoptosis and caspase substrate cleavage, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples.
Routine immune assays include multiplex Luminex, flow phenotyping of circulating white blood cells, and GeoMX digital spatial profiling. Small extracellular vesicles can be enriched from plasma, urine, and cell culture supernatant using various platforms (Izon, Clara Biotech, etc) depending on size and sample source
The services provided by the facility are tailored to suit experimental designs at various stages of development including basic science, preclinical, and translational research. This resource supports projects from investigators at the Sidney Kimmel Comprehensive Cancer Center, Thomas Jefferson University, and partners across the city in academia and industry.
Major Equipment
Flow Sorters
- BD Aria II high speed cell sorter: 5-Laser, 18-color (configuration)
- BD FACS Melody streamline cell sorter: 3-laser, 8-color (configuration)
Coming Soon
- Cytek Aurora CS Spectral cell sorter: 5 lasers, 64-channels, 40-colors
Flow Analyzers
- BD Symphony A5: 5-laser, 23-color (configuration)
- BD Symphony A3: 5-laser, 23-color (configuration)
Coming Soon
- Cytek Aurora Spectral analyzer: 5 lasers, 64-channels, 40-colors
Immune Monitoring
- Synergy H1 plate reader
- FM3D Luminex
- GeoMX digital spatial profiling
- Heska Element HT5+
Extracellular Vesicle Analyses
- ZetaView QUATT small particle characterizer
- Izon qEV small vesicle isolation platform
- Clara Biotech ExoRelease small vesicle isolation platform
Services
- Flow analysis workstations (PC/Mac)
- Flow panel design
- Training for new users
- Classes and webinars
- Consulting (experiment design and data analysis)
- Human sample collection, processing, and short-term storage (blood, urine, stool, tissue)
Upcoming Event Melanoma Patient & Caregiver Educational Meeting
Patients, caregivers and other attendees will learn the latest on research advances and treatment options for uveal melanoma. Presentations will be given by experts in the field, including, clinicians, researchers, and patient advocates. All will have the opportunity to ask questions. Available resources for patients and caregivers will be discussed and distributed. There will be breakout sessions where specific topics will be discussed.
When: Saturday, June 22, 8:00 AM – 8:00 PM (attendee arrival and late registration, 8:00 AM; meeting start, 9:00 AM)
Where: Dorrance Hamilton Building, Connelly Auditorium; 1001 Locust Street; Philadelphia, PA 19107