Confocal & Multiphoton Imaging
Missions, Goals, Capabilities
The confocal, multiphoton and super-resolution imaging facility will provide services particularly focused on high resolution fluorescence visualization of cells and tissue. The available confocal/multiphoton system provides for a range of experiments from sub-cellular localization to mapping of large tissue sections. The facility will provide access to this instrument for researchers, training on its use and consultation on experimental design and analysis.
Zeiss LSM780 NLO confocal/multiphoton microscope: 405, 457, 488, 514, 561, 594 and 633 nm laser lines for single photon excitation. Chameleon Vision II system (Coherent) for MP illumination. The confocal scanner is coupled to a Zeiss inverted microscope (Axio Observer). Three internal detectors including Meta detector for spectral imaging. Two external, non-descanned detectors for multi-photon imaging. Transmitted light detector for DIC/phase contrast. 10x, 20x, 25x, 40x, 63x and 100x objectives. Targeted illumination capability for photo-bleaching/activation; motorized focus for z-stacks; encoded, motorized stage; temperature and humidity controlled incubator with CO2 regulation. Zen acquisition and analysis software.
- High resolution, 3D imaging of live and fixed specimens
- Tile-scan images for large areas
- Imaging in multi-well formats
- Photo-activation, bleaching or other optogenetic techniques
- Tissue imaging with multi-photon excitation
- Training on microscope use and data analysis
- Consultation on experiment design.