Zeiss LSM780 NLO confocal/multiphoton microscope: 405, 457, 488, 514, 561, 594 and 633 nm laser lines for single photon excitation. Chameleon Vision II system (Coherent) for MP illumination. The confocal scanner is coupled to a Zeiss inverted microscope (Axio Observer). Three internal detectors including Meta detector for spectral imaging. Two external, non-descanned detectors for multi-photon imaging. Transmitted light detector for DIC/phase contrast. 10x, 20x, 25x, 40x, 63x and 100x objectives. Targeted illumination capability for photo-bleaching/activation; motorized focus for z-stacks; encoded, motorized stage; temperature and humidity controlled incubator with CO2 regulation. Zen acquisition and analysis software.
