Laser Capture Microdissection

Grant Information

Contact

Name: Raj Vadigepalli, PhD
Position: Director
Contact Number(s):

Contact

Name: Sirisha Achanta
Position: Technician
Contact Number(s):

Mission, Goals, Capabilities

Laser capture microdissection (LCM) is a technique that allows the precise procurement of enriched cell populations from a heterogeneous tissue under direct microscopic visualization. LCM can be used to harvest the cells of interest directly or can be used to isolate specific cells by ablating the unwanted cells, resulting in histologically enriched cell populations. 

The fundamental components of laser microdissection technology are (a) visualization of the cells of interest via microscopy, (b) transfer of laser energy to a thermolabile polymer with either the formation of a polymer–cell composite (capture method) or transfer of laser energy via an ultraviolet laser to photovolatize a region of tissue (cutting method), and (c) removal of cells of interest from the heterogeneous tissue section. Laser energy supplied by LCM instruments can be infrared (810 nm) or ultraviolet (355 nm). Infrared lasers melt thermolabile polymers for cell capture, whereas ultraviolet lasers ablate cells for either removal of unwanted cells or excision of a defined area of cells. LCM technology is applicable to an array of applications including mass spectrometry, DNA genotyping and loss-of-heterozygosity analysis, RNA transcript profiling, cDNA library generation, proteomics discovery, and signal kinase pathway profiling.

Major Equipment

ARCTURUS XT instrument with UV and IR laser enabled laser capture micro dissection.

ArcturusXT LCM Instrument

ARCTURUS XT instrument with UV and IR laser enabled laser capture micro dissection; 2X, 10X, 20X, 40X and 60X objectives; Nikon Phase Contrast Illumination, Microscope binoculars, epi-fluorescence illumination with red, green and blue filter cubes, Differential Interference Contrast with 10X, 20X, 40X and 60X DIC sliders, UV filter cube, triple dichroic filter set for DAPI/FITC/TRITC; Petri dish stage insert; AutoScan XT software.

Laser Capture Microdissection Services

1/2 day session - $100.00 | Laser Capture Microscopy Request Form

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